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Literature summary extracted from

  • de Veer, S.J.; Furio, L.; Swedberg, J.E.; Munro, C.A.; Brattsand, M.; Clements, J.A.; Hovnanian, A.; Harris, J.M.
    Selective substrates and inhibitors for kallikrein-related peptidase 7 (KLK7) shed light on KLK proteolytic activity in the stratum corneum (2017), J. Invest. Dermatol., 137, 430-439 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.117 expression in Pichia pastoris Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.117 GKCLFSEPPICFPN peptide inhibitor based on sunflower trypsin inhibitor-1 Homo sapiens
3.4.21.117 GKCLFSNPPICFPN peptide inhibitor based on sunflower trypsin inhibitor-1, displays at least 1000fold selectivity over several proteases that are related by function (KLK5 and KLK14) or specificity (chymotrypsin) Homo sapiens
3.4.21.117 GKCLFSSPPICFPN peptide inhibitor based on sunflower trypsin inhibitor-1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.117 0.044
-
 KHLY-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 0.048
-
 KHLF-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 0.049
-
 QHLY-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 0.65
-
 methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide pH 8.0, 23°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.117 Homo sapiens P49862
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.117 stratum corneum
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.117 KHLF-4-nitroanilide + H2O
-
 Homo sapiens KLHLF + 4-nitroaniline
-
 ?
3.4.21.117 KHLY-4-nitroanilide + H2O
-
 Homo sapiens KLHLY + 4-nitroaniline
-
 ?
3.4.21.117 methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O
-
 Homo sapiens methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
-
 ?
3.4.21.117 QHLY-4-nitroanilide + H2O
-
 Homo sapiens QLHLY + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.117 KLK7
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.117 1.2
-
 methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 7.4
-
 KHLF-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 7.7
-
 QHLY-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 12
-
 KHLY-4-nitroanilide pH 8.0, 23°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.117 0.00000014
-
 GKCLFSNPPICFPN pH 8.0, 23°C Homo sapiens
3.4.21.117 0.0000014
-
 GKCLFSSPPICFPN pH 8.0, 23°C Homo sapiens
3.4.21.117 0.0000031
-
 GKCLFSEPPICFPN pH 8.0, 23°C Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.21.117 physiological function inhibition of KLK7 alone is sufficient to block shedding, whereas KLK5 is also a major contributor Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.117 1.8
-
 methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 150
-
 KHLF-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 160
-
 QHLY-4-nitroanilide pH 8.0, 23°C Homo sapiens
3.4.21.117 270
-
 KHLY-4-nitroanilide pH 8.0, 23°C Homo sapiens